The Unit is using molecular genetic approaches to study the development of neuronal cell types, with a particular focus on neuronal arbor formation in retinal neurons. This Section is part of the Neurobiology Neurodegeneration & Repair Laboratory.
- Developing methodologies for sparse genetic labeling of neuronal arbors.
- Defining the transcriptional requirements for the formation of Retinal Ganglion Cell (RGC) types.
- Designing tools for physiological and behavioral analysis of normal and pathological vision.
Chuang K, Nguyen E, Sergeev Y, Badea TC. Novel Heterotypic Rox Sites for Combinatorial Dre Recombination Strategies. G3 (Bethesda). 2015 Dec 29;6(3):559-71.
Sajgo S, Ali S, Popescu O, Badea TC. Dynamic expression of transcription factor Brn3b during mouse cranial nerve development. J Comp Neurol. 2015 Sep 10.
Kretschmer F, Sajgo S, Kretschmer V, Badea TC. A system to measure the Optokinetic and Optomotor response in mice. J Neurosci Methods. 2015 Aug 14;256:91-105.
Sajgo S, Ghinia MG, Shi M, Liu P, Dong L, Parmhans N, Popescu O, Badea TC. Dre - Cre sequential recombination provides new tools for retinal ganglion cell labeling and manipulation in mice. PLoS One. 2014 Mar 7;9(3):e91435.
Shi M, Kumar SR, Motajo O, Kretschmer F, Mu X, Badea TC. Genetic Interactions between Brn3 Transcription Factors in Retinal Ganglion Cell Type Specification. PLoS One. 2013 Oct 8;8(10):e76347.
Badea TC, Williams J, Smallwood P, Shi M, Motajo O, Nathans J. Combinatorial expression of Brn3 transcription factors in somatosensory neurons: genetic and morphologic analysis. J Neurosci. 2012 Jan 18;32(3):995-1007. PubMed
Matsuoka RL, Chivatakarn O, Badea TC, Samuels IS, Cahill H, Katayama K, Kumar SR, Suto F, Chdotal A, Peachey NS, Nathans J, Yoshida Y, Giger RJ, Kolodkin AL. Class 5 transmembrane semaphorins control selective Mammalian retinal lamination and function. Neuron. 2011 Aug 11;71(3):460-73.
Chen SK, Badea TC (co-corresponding author), Hattar S. Photoentrainment and pupillary light reflex are mediated by distinct populations of ipRGCs. Nature. 2011 Jul 17;476(7358):92-5.
McNeill DS, Sheely CJ, Ecker JL, Badea TC, Morhardt D, Guido W, Hattar S. Development of melanopsin-based irradiance detecting circuitry. Neural Dev. 2011Mar 18;6(1):8. [Epub ahead of print]
Matsuoka RL, Nguyen-Ba-Charvet KT, Parray A, Badea TC, Chedotal A, Kolodkin AL. Transmembrane semaphorin signalling controls laminar stratification in the mammalian retina. Nature. 2011 Feb 10;470(7333):259-63.
Badea TC, Nathans J. Morphologies of mouse retinal ganglion cells expressing transcription factors Brn3a, Brn3b, and Brn3c: analysis of wild type and mutant cells using genetically-directed sparse labeling. Vision Res. 2011 Jan 28;51(2):269-79. Epub 2010 Sep 6.
Badea TC, Hua ZL, Smallwood PM, Williams J, Rotolo T, Ye X, Nathans J. New mouse lines for the analysis of neuronal morphology using CreER(T)/loxP-directed sparse labeling. PLoS One. 2009 Nov 16;4(11):e7859.
Badea TC, Cahill H, Ecker J, Hattar S, Nathans J. Distinct roles of transcription factors brn3a and brn3b in controlling the development, morphology, and function of retinal ganglion cells. Neuron. 2009 Mar 26;61(6):852-64.
Badea TC, Nathans J. Quantitative analysis of neuronal morphologies in the mouse retina visualized by using a genetically directed reporter. J Comp Neurol. 2004 Dec 20;480(4):331-51.
Badea TC, Wang Y, Nathans J. A noninvasive genetic/pharmacologic strategy for visualizing cell morphology and clonal relationships in the mouse. J Neurosci. 2003 Mar 15;23(6):2314-22.
Coronal section through the brain of a Brn3aCKOAP/+; Pax6 :Cre mouse. Retinorecipient areas receiving projections from RGCs expressing Brn3a are labeled in purple, and include the Medial Terminal Nucleus (MTN) of the Accessory Optic System, the Lateral Geniculate Complex (LGN) and the Pretectal Area (PA).
Dissecting the molecular mechanisms of dendritic arbor formation and axon guidance in RGCs.
Morphologies of mouse retinal ganglion cells visualized by sparse genetic activation of an alkaline phosphatase reporter targeted to the Brn3a, Brn3b, or Brn3c loci. Retinal ganglion cells were traced using Neuromantic software and are shown en face and in cross section. Colored boundaries indicate the morphologic types characteristic for retinal ganglion cells expressing the indicated Brn3 family member.
A. Dual pharmacological control of Cre-loxP recombination: In the ROSA26-rtTA-CreER knock-in line, rtTA is constitutively transcribed under the control of the ROSA26 promoter, activated by the addition of Doxycycline, binds the tetracycline response element (TRE) and induces transcription of CreER. CreER is then activated by 4HT, to induce recombination at target loci, with excision of an endogenous gene and expression of a reporter.
B. Retinal flat mounts from mice exposed to increasing amounts of Doxycycline and 4HT show increased frequency of recombination.
Tudor Constantin Badea, MD, PhD
Tenure Track Investigator
MA, Columbia University; MD, University of Medicine and Pharmacy (Romania); PhD Johns Hopkins University Medical School
Molecular genetic tools for combinatorial gene manipulation in mice. Developmental mechanisms of cell type specification. Retinal Ganglion Cell circuits. Mouse visual system analysis. More information at Dr. Badea's IRP page.
Manvi Goel, PhD
PhD, National Brain Research Centre (India)
I am studying the molecules that might have a role in synapse formation in the inner retina.
BS, Penn State University
As a future physician, I am interested in learning about the medical basis of degenerative eye diseases like AMD and glaucoma. Through my research, I hope to elucidate the molecular mechanisms of retinal ganglion cell development, as well as the effects of divergences in that development.
Vladimir Muzkya, PhD
BS, PhD, Novosibirsk State University
I am interested in nervous system development, cell type specification and mechanisms of neuronal function. In my research I utilize cell and molecular biology techniques, histology and anatomy, as well as imaging and work with small laboratory animals.
BA, University of Maryland, Baltimore County
The research that I am involved in is focused on the study of Retinal Ganglion Cells, the neuros that carry the visual information from the eye to the brain. We generate genetically labelled mouse lines that can specifically target individual RGC types, and then stain image and analyze the anatomic features of these cells.
Alumni /Former Lab Members
- Katherine Chuang
- Alirez Ghahari
- Miruna Ghinia
- Friedrich Kretschmer
- Sumit Kumar
- Oluwaseyi Motajo
- Eileen Nguyen
- Szilard Sajgo
- Ali Seid
- Melody Shi