Skip to content

Confocal Microscopy Unit

A confocal microscope image of a section of human retina, showing a specialized region called the fovea. Fluorescent probes have been used to identify cell nuclei (blue) and rod photoreceptors (green).

About our work

The Histology Unit is part of the Biological Imaging Core Facility.

High resolution fluorescence imaging of ocular tissues presents unique opportunities as well as technical challenges for scientists attempting to visualize pathological changes in the cornea, lens, retina or retinal pigment epithelium. Exploiting the transparency of the vertebrate eye and innovations in ocular imaging, vision researchers and ophthalmologists have gained unprecedented insight into a wide array of ocular processes.

 

Facilities and equipment

  • Leica SP8 Resonant Scanning Confocal Microscope + Spectra Physics Mai Tai “Deep See” Multi-photon Laser
  • Zeiss LSM 880 Confocal Microscope – 32 channel GaAsP + Airyscan detector for super-resolution imaging
  • Zeiss LSM 700 Confocal Microscope – 4 laser lines/ motorized stage/ live cell imaging
  • Olympus FV1000 Confocal Microscope
  • Zeiss Imager Z1 – Image acquisition of fluorescently labeled and H&E samples
  • Zeiss PALM Laser Capture Microdissection System
  • Phoenix Labs Micron III Rodent Fundus Imaging System

 

Services

  • Sample preparation for high resolution fluorescence imaging
  • Guidance in refining in vivo and in vitro imaging of ocular tissues
  • Training in image analysis and quantification
  • Rodent fundus imaging

Confocal Microscopy Unit key staff

Key staff table
Name Title Email Phone
Robert Fariss, Ph.D. Unit Chief farissr@nei.nih.gov 301-496-2829
Jinbo Li Histology Technician jinbo.li@nih.gov 301-402-9169
Noor D. White, Ph.D. Staff Scientist noor.white@nih.gov 301-402-5734

Last updated: January 11, 2023