Skip to content

NEI Researchers Awarded Grants by the Knights Templar Eye Foundation

July 11, 2019
7 men looking at the camera

From left: J. Fielding Hejtmancik, M.D., Ph.D., chief of the Ophthalmic Molecular Genetics Section; Sir Knight John H. Austin, Eminent Grand Generalissimo, Grand Commandery of Knights Templar of Maryland; Oussama M’Hamdi, Ph.D.; Robert Hufnagel, M.D., Ph.D.; Sir Knight William F. Reinhold, Right Eminent Mid-Atlantic Department Commander, Grand Encampment of Knights Templar; Sir Knight Spyridon G. Treklas, Right Eminent Grand Commander, Grand Commandery of Knights Templar of Maryland; and Brian P. Brooks, M.D., Ph.D.

The Knights Templar Eye Foundation has awarded two NEI scientists grants to research inherited retinal degenerations, diseases that can cause blindness in early childhood.

Robert Hufnagel, M.D., Ph.D., chief of the Medical Genetics and Ophthalmic Genomics Unit, and Oussama M’Hamdi, Ph.D., postdoctoral fellow in the Ophthalmic Molecular Genetics Section each received $65,000 toward their projects. The Knights Templar Eye Foundation, a charity sponsored by the Grand Encampment of Knights Templar, supports early career vision scientists who, like Hufnagel and M’Hamdi, study the biology of pediatric blinding conditions. Two other NEI scientists, Aman George, Ph.D. and Nathan Hotaling, Ph.D., have received grants from the Knights Templar Eye Foundation in recent years.

“Funding of young investigators is crucial to sustaining research,” said NEI Clinical Director Brian Brooks, M.D., Ph.D, who leads the Ophthalmic Genetics and Visual Function Branch, where the awardees work. “Generous funds today from the Knights Templar Eye Foundation is a downpayment on tomorrow’s cures for pediatric eye diseases.”

The health and maintenance of the retina, the light-sensitive tissue at the back of the eye, depends on coordination among its various cell layers. The light-sensing photoreceptors are nourished and supported by the adjacent retinal pigment epithelium (RPE) and choroid. Failure of one cell type can lead to retinal degeneration and subsequent blindness.

Robert Hufnagel, M.D., Ph.D. (left) and Oussama M'Hamdi, M.D. (right).

Oussama M’Hamdi, Ph.D. receiving award

From left: John Austin, Fielding Hejtmancik, Ph.D., Oussama M’Hamdi, Ph.D., William Reinhold, and Spyridon Treklas.

Robert Hufnagel, M.D., Ph.D. receiving his award

From left: John Austin, Brian Brooks, M.D., Ph.D., Robert Hufnagel, M.D., Ph.D., William Reinhold, and Spyridon Treklas.

Two men working in a lab

Robert Hufnagel, M.D., Ph.D. (right) with Temesgen Fufa, Ph.D. (left), in the laboratory.

M’Hamdi, who works in the laboratory of J. Fielding Hejtmancik, M.D., Ph.D., has recently identified mutations in a novel retinal gene that cause an inherited form of retinal degeneration, called autosomal recessive retinitis pigmentosa (RP). The mutations in these families cause loss of vision in infancy or childhood and early onset blindness.

“There’s very little known about this gene,” said M’Hamdi. “We were the first to find this protein in the retina, but we still don’t know exactly what it’s doing.”

M’Hamdi believes that the mutated gene functions in the photoreceptor, providing support for the membranous disks containing rhodopsin in the outer segments. M’Hamdi is using cell culture systems as well as zebrafish and mice to study the gene’s role in photoreceptor function and survival.

Hufnagel is studying how the photoreceptors and RPE communicate. Mutations in the protein neuropathy target esterase (NTE) can lead to a syndrome known as Oliver-McFarlane syndrome, which causes retinal degeneration in childhood. NTE is important for maintaining cellular membranes. He proposes that NTE’s function might extend to the formation of membrane-enclosed vesicles, also called exosomes, that ferry proteins and metabolites between cells. Hufnagel believes that exosomes transport factors crucial for the health of photoreceptors from the RPE, and that impairment of this process may lead to vision loss in Oliver-McFarlane syndrome and other pediatric retinal degenerations. His Knights Templar grant will support Postdoctoral Fellow Takerra Johnson, Ph.D., who will investigate the formation and composition of exosomes in the retina.

“We’ve known for a long time that the retinal pigment epithelium maintains the function of the photoreceptors by recycling their outer segments and completing the visual transduction cycle,” said Hufnagel. “Less clear is how else the RPE, photoreceptors, and the choroid communicate and support each other to maintain function of the retina. That’s what we’re looking into.”


This press release describes a basic research finding. Basic research increases our understanding of human behavior and biology, which is foundational to advancing new and better ways to prevent, diagnose, and treat disease. Science is an unpredictable and incremental process— each research advance builds on past discoveries, often in unexpected ways. Most clinical advances would not be possible without the knowledge of fundamental basic research.

NEI leads the federal government’s research on the visual system and eye diseases. NEI supports basic and clinical science programs to develop sight-saving treatments and address special needs of people with vision loss. For more information, visit

About the National Institutes of Health (NIH): NIH, the nation’s medical research agency, includes 27 Institutes and Centers and is a component of the U.S. Department of Health and Human Services. NIH is the primary federal agency conducting and supporting basic, clinical, and translational medical research, and is investigating the causes, treatments, and cures for both common and rare diseases. For more information about NIH and its programs, visit


Lesley Earl